Description
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GFP in biological agents.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to GFP. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to GFP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GFP, biotin-conjugated antibody and enzymeconjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GFP in the samples is then determined by comparing the O.D. of the samples to the standard curve